NAME________________________ DATE_____________ HOUR____________

NAME________________________ DATE_____________ HOUR_____________

BIOLOGY ONE

POND WATER ADVENTURE

MICROSCOPE – 4

DIRECTIONS: Make a wet mount of a sample of pond water. Observe them under the microscope and sketch as many organisms as possible. HINT – Use low power…

MAGNIFICATION_______ ESTIMATED SIZE_______	MAGNIFICATION_______ ESTIMATED SIZE_______
MAGNIFICATION_______ ESTIMATED SIZE_______	MAGNIFICATION_______ ESTIMATED SIZE_______
MAGNIFICATION_______ ESTIMATED SIZE_______	MAGNIFICATION_______ ESTIMATED SIZE_______

QUESTIONS AND ANALYSIS

Describe any difficulties encountered while finding the microorganisms with the microscope.

Was anything found that looked like something alive but really it was not? What made it appear to be alive?

Describe for someone who has never used a microscope before how to quickly find something on a slide.

Describe each of the organisms that you found. What were they doing, how were they moving…

One thing that I was surprised to learn about microscopic life was:

Using the following information, answer these questions about estimating the size of microscopic measurements.

ALL MEASUREMENTS ARE IN MICRONS (m)

MAGNIFICATION	WIDTH OF FIELD OF VIEW	POINTER LENGTH	POINTER WIDTH AT TIP
100X	1700	160	50
150X	1130	110	33
200X	850	80	25
300X	570	50	17
400X	420	40	12
500X	340	32	10

I am using 200X. The worm I am looking at is half as long as the field of view. How long is it in microns?

I find the nucleus of a cell under high power (500X) It is twice as wide as the pointer tip. How wide is it?

I am looking at a rectangular looking plant cell under 400X. It is almost as long as the pointer, and almost twice as wide as the pointer tip. Estimate its length and width.